What Are the Different Types of Microscopy Methods?

Updated on October 19, 2022

There are several different types of microscopy. These include Light sheet microscopy, Fluorescence microscopy, and Atomic force microscopy. Each method is unique and can be used for other purposes. Here are some differences between the various types of microscopy.

Light Microscopy

Light microscopy is a method that uses a thin sheet of light that is projected onto a sample. The imaging system is located in the same plane as the specimen. This allows for fast and high-quality imaging while minimizing the risk of photodamage. Its development traces its roots to the single-plane illumination microscopy (SPIM) system. However, it has evolved into many different configurations over the years.

Light sheet microscopy can be used to image a variety of biological samples. This technique is beneficial for intracellular imaging processes and subcellular structures. It is also valuable for long-term imaging of model systems. This method can also visualize macroscale samples, and Microvision Labs can assist you in conducting this service.

It has the advantage of being highly sensitive. Its dual-sided illumination minimizes shadow artifacts and dark lines that appear across a large specimen. It also offers high resolution and signal-to-noise ratios.

Electron Microscope

Electron microscopes produce images by probing samples using electrons instead of light. The electron beam travels through the microscope’s lenses and electromagnetic fields. As it interacts with the sample, it loses energy. The energy is converted into high-energy backscattered and secondary electrons, which help determine crystal structures and orientations. X-rays and visible light create images, but backscattered electrons provide the most detailed information about the sample surface.

For electron microscopy to work, specimens must be fragile. They must be between 20 and 100 nanometers thick. This ensures the beam can pass through the specimen without leaving any voids. Staining the model with materials dense in electrons enhances the image quality. Several requirements are necessary before using a TEM. These include a vacuum and a very thin and dehydrated sample. To get the most accurate images, the specimen must be prepared before it is placed in the microscope. Hiring the #1 web designers in West Palm Beach is a great advantage to expand the network and offer these services.

Fluorescence Microscopy

Fluorescence microscopy is a type of optical microscope. Instead of reflection, attenuation, and scattering, it uses light’s fluorescence to examine the properties of organic substances. It is beneficial for studying substances that emit or absorb light. The method is a popular choice for researchers studying biological systems.

Fluorescence is a phenomenon that occurs naturally in specific biological tissues. It is caused by photons absorbing light and then attaching to molecules that are of interest. An excellent example of a fluorescent molecule is DAPI, which can pass through a cell membrane and bind strongly to DNA’s adenine-thymine region. The choice of fluorescence molecule depends on the assay conditions, cell type, and part.

Fluorescence microscopy relies on the principle of fluorescence, which explains the resulting high-energy emission of light by matter. Fluorescent molecules absorb short-wavelength light and then excite as the light passes through them. This exciting process is also known as light-triggering fluorescence.

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